Custom Anti-Hapten Antibody Discovery Services
Combine years of experience in the development of anti-hapten antibodies and our capacity in hapten design, Creative Biolabs offers custom services for the development of anti-hapten monoclonal and polyclonal antibodies, which are particularly suited to diagnostic and theranostic applications.
Anti-hapten Antibody Development Services
The generation of monoclonal or polyclonal antibodies against hapten targets has some problems not encountered with larger antigens. Small molecules are low molecular weight molecules of less than 2-5 kDa and not immunogenic because they lack epitopes that could be recognized by T cells to elicit an immune response, even in the presence of adjuvant by their nature. They do not directly elicit an immune response until and unless conjugated with suitable carrier macromolecules that are immunogenic and they are termed as haptens when attached to large carrier proteins. By this means, these hapten-carrier conjugates could induce strong immune responses more efficiently and produce antibodies that bind to haptens with high-affinity. The successful production of desired antigen-special antibodies depends on multiple factors, including but not limited to the design and modification of hapten, selection of the appropriate carrier protein and the method of conjugation.
- Design, Synthesis, and Identification of Hapten
- Hapten-Carrier Conjugation and Identification
- Generation of Monoclonal or Polyclonal Antibody
- Antibody Purification and Labeling
- Antibody Application
Due to the immuno-chemical properties of the small molecule haptens such as the chemical structure, composition, immunogenicity, and analogues, Creative Biolabs can develop the most suitable specific haptens based on our advanced hapten design platform, further to maximize the antibody response required to trigger.
Haptens are non-immunogenic small molecules and usually need conjugating to carrier proteins, such as BSA (bovine serum albumin) and KLH (keyhole limpet hemocyanin) in order to expose the small molecule epitope in some ways. Besides, haptens are administered with adjuvants to ensure a high-quality immune response further.
Creative Biolabs offers two main strategies to facilitate the development of monoclonal or polyclonal antibodies, the establishment of our antibody development platform depends on hybridoma and phage display technology. It mainly includes: (1) the screening of hybridoma cells, from the immunization, fusion, and screening to subcloning and generation antibodies; (2) phage display technology, from the immunization, screening, and characteristic to generation antibodies.
Based on our broad range of available technologies, we can also provide antibody purification and production, antibody engineering, and antibody modification services to ensure high reproducibility, availability, and scalability.
The principal interest in these available anti-hapten antibodies with high quality can be served as detection reagents in diagnostic kits for diagnostic assays. They could be generated against various small molecules, including antibiotics, toxins, pesticides, antiparasitics, hormones, and food additives. These antibodies are essential in serology, drug delivery, development of diagnostic kits, or biosensors, such as for the quantification, detection, and monitoring of haptens in the samples based on enzyme-linked immunosorbent assay (ELISA) method.
Features of Anti-Hapten Antibody Production Service
- A personalized strategy for antigen design and synthesis
- Multiple host animal species available
- Versatile strategies available for customers’ choices
- High affinity and specificity antibody production
- Fast turnaround time and quality controls at each phase
- Competitive pricing
- Timely progress reports
Anti-hapten antibodies are attractive tools both in the field of food safety and environmental monitoring as well as the human disease diagnostic and therapeutic applications. Creative Biolabs provides custom services for anti-hapten monoclonal and polyclonal antibody for our global clients. If you are interested in our service, please feel free to contact us for detailed information.