Design and Synthesis of Compound-specific Haptens for Benzimidazoles
Benzimidazoles (BZs) are a class of drugs with activities against fungi, protozoa, and helminths and are commonly employed in veterinary and human medicine. BZ drugs have many benefits, including selectivity, low mammalian toxicity, high efficacy, ease of administration and low cost. However, the overusing or/and losing control of uses of BZs results in the emergence of BZ resistance. In order to monitor and control the usage of BZs, antibodies against BZs are needed. Based on many years of professional experience in the design and development of small molecule antibodies, Creative Biolabs offers the design and synthesis of compound-specific haptens for BZs to support the relative researches and detections by the immunoassay methods. Furthermore, our service also contains the modification and characterization of haptens based on customers’ special objectives.
Design and Synthesis of Compound-specific Haptens for Benzimidazoles
For compound-specific haptens design, we mainly focus on the position in C5 of BZs, shown in Fig.1.
Fig.1 The position in BZ for the compound-specific haptens design and synthesis.
- Haptens design.
- Hapten modification.
- Choice of linking spacer in hapten derivatives.
- Preparation of hapten derivatives.
- Haptens conjugation
The molecular structure of hapten should contain a certain degree of complexity or rigidity, such as containing benzene ring, heterocyclic group, branch structure and so on.
Haptens might be directly coupled to the carrier for the preparation of artificial antigen if it contains active groups, such as –COOH, –NH2, –OH and so on.
The linking between the hapten and carrier is the linking spacer. The selection of linking spacer should be fellow the two principles:
(1) The linking spacer should be far from the functional groups to avoid affecting the recognition of the antibodies to hapten.
(2) The length of the linking spacer should be suitable.
Hapten derivatives can be prepared via modifying the existing hapten, relevant intermediate or metabolite of the hapten, or be re–synthesized starting from raw materials.
A variety of carriers can be employed for coupling with haptens. The most frequently used carriers include serum globulins and albumins, keyhole limpet-hemocyanin (KLH), gelatin, ovalbumin, casein, hemocyanin, thyroglobulin, fibrinogen, and tetanus, cholera, or diphtheria toxoid. It is worthy to be noticed that conjugates can be injected into any animal except the animal of origin for the carrier protein when used for the production of antibodies.
At Creative Biolabs, we provide the design and synthesis of compound-specific haptens for BZs with outstanding researchers and advanced technologies. We also offer the design and synthesis of class-specific haptens for BZs. If you want to know more information, please feel free to contact us.