Design and Synthesis of Class-specific Haptens for Benzimidazoles

Benzimidazoles (BZs) are a large chemical family employed to treat nematode and trematode infections in domestic animals. The success of anthelmintic treatment within the management and control of parasitic infections among livestock in the years following the development of BZ causes frequent and indiscriminate use of these drugs. In order to monitor the use and dose level of BZs, an easy-to-use and economical detection method is necessary for environmental and agricultural trace analyses. Immunoassays meet these requirements. In immunoassays, researchers need firstly to develop antibodies as tools. Combining many years of experience in designing and synthesizing small molecule antibodies, Creative Biolabs’ professional teams offer the design and synthesis of class-specific haptens for BZs. Moreover, the service also includes the modification and characterization of haptens for BZs based on customers’ special requirements.

Advantages of Haptens

In general, small chemical compounds, haptens (700–1500 Da), are small molecules that can stimulate the production of the antibody when conjugated to a carrier molecule. Because of the small size, haptens cannot be presented to T cells since they cannot bind to major histocompatibility complex (MHC) independently and trigger the immune response significantly. However, the haptens can be combined with the carriers, which are relatively larger molecules traveling through the circulation but do not induce the immune response by themselves, such as keyhole limpet haemocyanin (KLH) and bovine serum albumin (BSA), the resulting hapten-carrier conjugates will take advantage of the larger size and then trigger the immune response more efficiently.

Design and Synthesis of Class-specific Haptens for Benzimidazoles

For class-specific haptens development, we mainly focus on three positions in BZs, including N1, C2, and R2, shown in Fig.1.

• Select the most suitable position on the molecule to attach a linker based on customers’ requirements. The position with the linker will improve the specificity, sensitivity and immunogenicity of the immunogen.
• Design and synthesize the most proper linker. The choice of linker should be fellow the two basic principles. The first one is the linker should be far from the functional groups to avoid affecting the recognition of antibodies to hapten. The second one is the length of the linker should be appropriate.
• Immunogen characteristics and Optimization.

Figure.1 The positions in BZ for the class-specific haptens design and synthesis.

Creative Biolabs is co-located in mixed discipline labs to facilitate effective communication and problem-solving. Creative Biolabs has rich experience in antibody development and can provide customers with one-stop services including hapten design, modification, synthesis, characterizations, as well as subsequent antibody production. Our advanced technologies and experienced researchers are committed to improving your program and decreasing the overall development timeline. Also, we provide design and synthesis of compound-specific haptens for BZs. For more information, please don't hesitate to contact us.