Design and Synthesis of Haptens for Sudan I
As one of the top CRO companies in the field of small-molecule antibody development for disease therapy, Creative Biolabs has focused on hapten studies for years and has accomplished numerous challenging projects for our valued clients. Equipped with deep knowledge and experience in hapten design, we can offer high-quality custom hapten solutions for Sudan I.
Introduction to Sudan Dyes
Sudan dyes are a group of synthetic additives that has been broadly used in a number of industrial and scientific applications, such as oils, food, as well as plastics. There are four types of Sudan dyes, including Sudan I, Sudan II, Sudan III, and Sudan IV, which have been identified and exclusively studied in the past few years. Numerous data have demonstrated that Sudan dyes are associated with the occurrence and development of various human diseases, especially for cancers. As a result, Sudan dyes have been banned in most countries around the world. Meanwhile, a series of antibody-based immunoassays, such as enzyme-linked immunosorbent assay (ELISA), have been generated for detecting the presence of Sudan dyes in food products. In a recent study, a carboxyl on the benzene ring has been added for modifying the Sudan I to obtain suitable haptens for Sudan I or its derivatives. Moreover, the spacer length and coupling position of novel haptens have also been optimized to improve the antibody performance in clinical use.
Fig.1 Structures of Sudan dyes. (Rebane, 2010)
Our Platform for Design and Synthesis of Haptens for Sudan I
The illegal use of Sudan dyes as food additives can cause many severe side effects in humans. Therefore, the detection of Sudan dyes in different kinds of food samples has aroused much attention in recent years. In Creative Biolabs, we have successfully generated a well-mature platform to provide a wide spectrum of Sudan I hapten design and synthesis services for our clients. For instance, several haptens for Sudan I, hapten-1, hapten-2, and hapten-3, have been generated by modifying the hydroxyl group of Sudan I. A variety of coating antigens, such as bovine serum albumin (BSA) and ovalbumin (OVA) have been used for the development of new small-molecule antibodies against Sudan I or its residues. Besides, several assays have been recently established for assessing and optimizing the specificity and sensitivity of these haptens, as well as the cross-reactivity of antibodies against Sudan I derivatives. The results suggested that these antibodies have high specificity and high cross-reactivity (≥ 100%) in heterologous assays.
Creative Biolabs is a leader in the field of hapten design and small-molecule antibody discovery for years. With our extensive experience and advanced platform, we can provide a series of Sudan I-based hapten services basing on a wide variety of state-of-art technologies. We are proud to partner with our clients on the journey of bringing novel haptens to different clinical applications. If you are interested in our services, please contact us for more details. Let us know what you need and we will accommodate you. We look forward to working with you in the future.
Reference
- Rebane, R.; et al. A review of analytical techniques for determination of Sudan I-IV dyes in food matrixes. Journal of Chromatography A. 2010, 1217(17): 2747-2757.