Design and Synthesis of Haptens for Bacitracin
Creative Biolabs is dedicated to providing the first-class service of small molecule hapten synthesis and design to our customers around the world. We have developed a top hapten design and synthesis platform for anti-hapten antibody preparation. Here, we are happy to introduce our hapten design and synthesis service for bacitracin.
Introduction of Bacitracin
Bacitracin is an antibiotic isolated by the nutrient solution of Bacillus licheniformis or Bacillus subtilis mutation. With similar antimicrobial spectrum to penicillin G, bacitracin has a stronger restraining effect on gram-positive microorganisms and is mainly used in treating penicillin-fast staphylococcal infections. In addition, bacitracin has been also used as fodder additives in modern animal husbandry. As a consequence, residual bacitracin in milk and edible animal tissues might lead to the allergies and emergence of resistant microbes. Therefore, it is of great importance to detect bacitracin residues. Currently, detection methods such as immunoassays, liquid chromatography with ultra-violet, fluorescence detection, and mass spectrometry have been applied for the detection of bacitracin in commercial pharmaceuticals, feeds, and milk samples. Among these approaches, specific antigen-antibody interaction-based immunoassays represent a simple, accurate, and sensitive method that provides a means for rapid and efficient screening of the samples for bacitracin residue detection.
Fig.1 Structure of bacitracin.
Development of Bacitracin Hapten
Designing of antibodies plays a very important role in the efficiency enhancement of the screening assay, especially in the antigen-antibody immunoreactive immunology-based approach. However, bacitracin is micromolecular compound that does not have immunogenicity, which means macromolecular carrier albumen must be coupled with bacitracin to obtain immunogenic artificial antigen and thus produce specific antibodies. It has been reported that bacitracin could be used as a hapten to prepare a complete antigen by coupling with common carrier albumen, such as bovine serum albumin (BSA), and ovalbumin (OVA), human serum albumin (HSA), mouse serum albumin (MSA), thyroprotein (TG) or hemocyanin (KLH), etc. Specific monoclonal antibody obtained by immunizing the animal with this antigen has been proved can be applicable to detect bacitracin with higher sensitivity.
To promote the development of rapid and convenient immunoassays for the detection of drug residues, Creative Biolabs has established an advanced hapten design and synthesis platform to offer custom services for hapten development. With years of experience, our experienced scientists could propose professional advice on hapten modification and carrier protein selection. Moreover, we also provide one-stop antibody preparation, engineering, and labeling services for global customers.
Features of Our Services
- Advanced technology platforms for hapten design and modification
- Experienced staff and sophistically operating
- High-quality products and fast delivery
- Customized experimental design and professional technical support
Creative Biolabs has years of experience in the field of hapten design and synthesis and is an expert in producing specific antibodies to support both basic research and discovery projects of our clients. We pursue to provide products with optimal formats and cost-effective prices. For more detailed information, please feel free to contact us and consult our technical supports for more details.