Design and Synthesis of Compound-specific Haptens for Nitroimidazoles

Creative Biolabs has provided customers with countless high-quality technical services over the past ten years with its unique advantages in small molecule drug research and antibody development. For the development of antibiotic antibodies, we can provide customers with the design, modification and synthesis of compound-specific haptens for nitroimidazoles.

Residual drugs from edible livestock and poultry products are known as the biggest pollution problem in animal husbandry today, and these drug residues can cause different degrees of harm to consumers' health. Antibiotics are often added to livestock production to improve production efficiency. These antibiotics will remain in the animal body, and then accumulate in the human body ingesting animal products, resulting in an increase in human resistant bacteria. Some antibiotics and metabolites also have carcinogenic and teratogenic effects.

Nitroimidazole (NDZs) are one of the representatives of such antibiotics. The detection methods of antimicrobial residues in food are mainly divided into three methods: instrument method, immunological method and microbial detection method. Among them, the immune method represented by enzyme-linked immunosorbent assay method (ELISA) is a technology developed by combining the immune response of antigens and antibodies with the efficient catalytic reaction of enzymes. ELISA has the advantages of fast, strong specificity, high sensitivity, can detect multiple samples at the same time, and simple operation.

Design and Synthesis of Compound-specific Haptens for Nitroimidazoles

NDZs are a class of drugs with a 5-nitroimidazole ring structure. They include metronidazole (MNZ), dimethyl metronidazole (DMZ), ipronidazole (IPZ), and seconidazole (SCZ), ornidazole (ONZ), tinidazole (TNZ), lonidazole (RNZ), etc. Among them, DMZ and RNZ can be hydrolyzed to hydroxy metronidazole, MNZ is hydrolyzed to hydroxy metronidazole, and IPZ is hydrolyzed to hydroxypropidazole. Because NDZs are a huge category, it is a trend to detect as many NDZs in a sample as possible at the same time. However, due to the general structure of NDZs class-specific haptens, false positive results may also occur in the test results. In addition, in some cases, only a specific NDZs need to be detected. In these situations, it is necessary to design compound-specific NDZs haptens.

Structures of several NDZs drugs. Fig.1 Structures of several NDZs drugs.

The basic principle for the design of haptens of compound-specific NDZs antibody is to take the structure of the given compound as the backbone, and use mutual molecular field analysis (CoMFA), comparative molecular similarity indices analysis (CoMSIA) and other methods to model and analyze the interaction between haptens / antibodies, and then optimize and modify the hapten’s structure to explore the specificity and affinity of haptens to antibodies.

Creative Biolabs is proficient in applying a variety of computer simulation techniques and successfully applying them to antibody development practices. For the development of NDZs' compound-specific antibodies, we can provide first-class hapten design, transformation and synthesis services, and can assist customers in subsequent antibody preparation and evaluation.

If additional help is needed, please directly contact us and consult our technical supports for more details.

All listed services and products are for Research Use Only. Do Not use in any diagnostic or therapeutic applications.


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