Design and Synthesis of Class-specific Haptens for Anilinopyrimidine Fungicides

Creative Biolabs is a contract research organization (CRO) dedicated to promoting the development of anti-hapten antibody for the detection of small molecules. Benefiting from our expertise in small-molecule hapten design and synthesis, we can harness science and technology to design and synthesize class-specific haptens that support the next generation of broad-spectrum antibodies.

Anilinopyrimidine Fungicides Residues Detection

Anilinopyrimidine fungicides, including cyprodinil, mepanipyrim, pyrimethanil, and cyclopyrimidine, are widely used pesticides whose residues are commonly found in fruits and vegetables. Fungicide residues are usually analyzed by chromatographic multianalyte methods, such as high-performance liquid chromatography (HPLC) with a diode array detector, liquid chromatography with tandem mass spectrometry, or gas chromatography with mass spectrometry. Even though sensitive and accurate, these techniques are time-consuming and expensive. Currently, immunoanalytical techniques are wide-spread in various spheres of investigations. Among these immunoanalytical techniques, ELISAs are of great advantages like simplicity, high sensitivity, portability, affordable cost, and high sample throughput. Particularly, multianalyte competitive ELISAs have been developed for the recognization of several compounds simultaneously.

Molecular structures of pyrimethanil, cyprodinil, mepanipyrim, and hapten AP. Fig.1 Molecular structures of pyrimethanil, cyprodinil, mepanipyrim, and hapten AP. (Esteve-Turrillas, 2017)

class-specific haptens Development for Anilinopyrimidine Fungicides

Generally, multianalyte competitive ELISAs are developed based on specific or broad-specificity antibodies recognizing simultaneously several compounds. To achieve this goal, a rational design of haptens is needed for the preparation of immunogens. Pyrimethanil, cyprodinil, and mepanipyrim have a very similar molecular structure, with different substituent groups at the C-3 position of the pyrimidine ring. Based on a similar structure, a novel hapten molecule was designed by introducing a functional carboxylate group at the C-3 position. Then, the hapten was employed in the preparation of high-affinity antibodies class-specific to the anilinopyrimidine family of fungicides. Moreover, high-sensitivity immunoassays were developed to recognize simultaneously pyrimethanil, cyprodinil, and mepanipyrim. And later results have shown that the antibody provided an equal recognition of each anilinopyrimidine compound, with cross-reaction values close to 100%.

Class-specific Haptens Design and Synthesis Services Provided by Creative Biolabs

With rich experience in antibody development, Creative Biolabs is capable of offering one-stop anti-hapten antibody development services such as class-specific hapten design, modification and synthesis, and subsequent antibody production and labeling to satisfy different customers’ requirements. Our professional scientists are happy to take part in each step of your projects and promote the development of your research.

Features of Our Services

  • Highly efficient
  • One-stop service with customized proposals
  • Skillful scientific team with best after-sale service
  • Short turnaround time

By continuing to grow in response to the requirements of our clients, Creative Biolabs is dedicated to exploring novel and innovative technologies to offer the most comprehensive, integrated portfolio of anti-haptens antibody development. In addition to class-specific haptens, we also offer compound-specific haptens design and synthesis services to help our clients develop specific antibodies for the detection of numerous small molecules. If you are interested in our services, please feel free to contact us for more details.

Reference

  1. Esteve-Turrillas, F.A., et al. A class-selective immunoassay for simultaneous analysis of anilinopyrimidine fungicides using a rationally designed hapten. Analyst. 2017, 142(20), pp.3975-3985.
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