Design and Synthesis of Haptens for Tylosin
Tylosin is a macrolide antibiotic, which is obtained from the culture solution of Streptomyces macrobes and is widely used to treat bacterial infections in various animals. Its antibacterial spectrum is extensive, and it has a good inhibitory effect on some Gram-negative bacteria, mycoplasma, rickettsia and chlamydia. With the development of immune technology, small molecule compounds like tylosin have played an important role in immune tests related to environmental testing and food analysis. Creative Biolabs provides customers with tylosin hapten-related services through scientific synthesis schemes and modification methods.
Fig.1 Structure of Tylosin.
Tylosin Hapten Design and Synthesis Service
In order to obtain an antibody with sufficient titer, the molecular structure of the small molecule hapten needs to have a certain degree of complexity or rigidity, such as containing a benzene ring, a heterocyclic group, and a branched chain structure. Existing experiments show that the presence of benzene rings can greatly increase the success rate of antibody production. A hapten containing an active group can be directly coupled to a carrier without affecting its functional group to prepare an artificial antigen.
The basic principle of Creative Biolabs hapten design and synthesis is to keep the original molecular structure of hapten as much as possible, and expose the hapten to the surface of the carrier as much as possible, so that after immunizing animals with artificial antigen, the hapten can be recognized to the greatest extent, and stimulate animals to produce specific immune response, and obtain antibodies with high affinity.
Tylosin Hapten Modification Service
One of the advantages of Tylosin haptens is that they are relatively rich in modification sites. Hapten derivatives modified at different sites have different spatial structures exposed to artificial antigens, resulting in different titer, affinity and specificity of antibodies. Creative Biolabs focused on exposing the characteristic structure of haptens when modifying them to improve the success rate of antibody induction. In order to induce highly specific antibodies, the sites of hapten modification are far away from the characteristic structure and functional groups of the hapten, so that the characteristic structure of the hapten can be fully exposed, thus avoiding the shielding effect of carrier proteins. Our comprehensive hapten custom services cover a whole process from hapten design, hapten modification, to carrier selection and coupling reaction.
- Hapten design (e.g. preparation of hapten derivatives);
- Hapten modification (e.g. the choice of modified sites);
- Selection of carrier and coupling reaction (e.g. coupling methods and conditions);
- Purification of artificial antigens.
If you want to inquire more about our Tylosin hapten design and synthesis services, please contact us.