Design and Synthesis of Compound-specific Haptens for Quinolones
Creative Biolabs is second to none in the field of small molecule antibody development. In more than a decade of service history, we have helped thousands of customers successfully develop highly effective antibodies against various antibiotics, toxins, pesticides, heavy metals and food additives, etc. Our excellent service has won high recognition from customers.
The use of immunoassay technology for the detection of drug residues in animal-derived foods has become the current trend of drug residue analysis due to its simplicity and large-scale operation. As broad-spectrum antibacterial drugs, quinolone drugs are widely used in the prevention and treatment of animal diseases. Therefore, the detection and analysis of QNs is a major content of detecting harmful residues in animal products. At present, the design of most haptens is still based on experience. Designing a variety of haptens with different structures, and then obtaining antibodies after a long period of animal immunization, and then verifying the feasibility of the hapten, this method takes a lot of time, and often the specificity is not strong. The unclear recognition mechanism between antibodies and target analytes or small molecule compounds is the main cause of this problem.
Design and Synthesis of Compound-specific Haptens for Quinolones
In-depth research on the recognition mechanism of antibodies and target analytes or small molecule compounds has important guiding significance for designing and modifying haptens. The use of molecular simulation methods to explore the recognition between small molecule haptens and antibodies is a tool that is developing rapidly and highlighting its strong power. The quantitative structure-activity relationship (QSAR) is based on the analysis of existing active molecules, taking the structure and physical and chemical parameters of the compound as independent variables, and biological activity as the dependent variable. And use mathematical statistical methods to establish the quantitative relationship between the chemical structure and biological activity of a compound. By revealing the rule that the biological activity of a compound changes with the structure and physical and chemical parameters of the compound, it is possible to infer the mechanism of action of the compound and explain the recognition ability of antibodies and different compounds.
The configurational characteristics of quinolone drugs determine the specificity of antibody preparation. Creative Biolabs uses comparative molecular field analysis (CoMFA), comparative molecular similarity indices analysis (CoMSIA), distance geometric three-dimensional quantitative structure-activity relationship, molecular shape analysis, virtual receptors and many other methods to perform the in-depth research of interaction between antigen and antibody. Based on this guidance and by modifying existing haptens, a variety of haptens specific to a certain compound were designed and synthesized. Antibodies immunized with these haptens have good specificity and can meet the requirements for specific detection of certain analytes.
Taking flumequine as an example, it is the second generation of fluoroquinolones (FQs) developed in the 1970s and is still widely used today. Based on the flumequine skeleton, the corresponding hapten was synthesized by analyzing its interaction with antibodies and modifying its structure. Couple it with a carrier protein and immunize the animal, the antibody produced has less than 0.1% cross-reactivity with other FQs.
Harnessed with substantial experience in antibody development, Creative Biolabs is offering top-class design, modification and synthesis services for compound-specific haptens for quinolones. If additional help is needed, please directly contact us and consult our technical supports for more details.