Design and Synthesis of Class-specific Haptens for Nitroimidazoles

Creative Biolabs is the world's leading expert in antibody development. Our antibody development services cover a wide range of bio-macromolecule and small-molecule chemical drugs, as well as antibiotics, toxins, heavy metals and food additives.

Introduction to Nitroimidazoles

Nitroimidazole (NDZs) antibiotics are commonly used in livestock and poultry feeds for anti-protozoal infections and antibacterials. They have a growth-promoting effect and can also be used in combination with other antibiotics to treat anaerobic infections. However, NDZs and some of their metabolites are carcinogenic, teratogenic, mutagenic, and genotoxic to mammals, and are therefore classified as illicit drugs in many countries. Many countries require the detection of NDZs residues and their metabolites in animal-derived foods, among which metronidazole, 1- (2-hydroxyethyl) -2-hydroxymethyl-5-nitroimidazole, dimenidazole, hydroxymethyl metronidazole, cemidazole, tinidazole, lronidazole, clonidazole, benzimidazole and the like. Enzyme-linked immunosorbent assay (ELISA) has the advantages of simplicity, fastness, sensitivity, and low cost, and has been widely used in the detection of chemical residues such as pesticides.

Design and Synthesis of Class-specific Haptens for Nitroimidazole

The key to a successful ELISA method is to obtain a highly potent and high specificity antibody. Because of the variety of NDZs, to detect multiple NDZs in one sample and avoid adding multiple NDZ antibodies at the same time, it is necessary to develop class-specific NDZs antibodies. This involves the design of class-specific haptens. One of the strategies is to analyze the chemical structure of NDZs, and use the general structure of 2-methyl-5-nitroimidazole as the backbone to design haptens, and select suitable carrier proteins and ligation methods to prepare theoretically available complete antigens. Furthermore, antibodies capable of recognizing various NDZs having a 2-methyl-5-nitroimidazole structure were produced by immunizing animals with the above designed complete antigen. The results show that the recovery rate of the ELISA method based on the NDZs antibodies prepared by this strategy is between 85% and 100%. The prepared antibody reacted well to five NDZs of 2-methyl-5-nitroimidazole, metronidazole, tinidazole, dimenidazole, and seclidazole and their metabolites, with a cross-reaction rate of more than 95% and a detection limit of approximately 1.9 ×10-4 mg/L.

The core structure of many nitroimidazole antibiotics. Fig.1 The core structure of many nitroimidazole antibiotics.

Comprehensive use of computer simulation technology and small molecule compound antibody development experience, combined with existing NDZs hapten design strategies, and optimization and improvement, Creative Biolabs can provide customers with complete NDZs class-specific hapten design, modification and synthesis services.

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All listed services and products are for Research Use Only. Do Not use in any diagnostic or therapeutic applications.

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